1. The role of each structure fermenter
1.1 precision filter is generally used for a period of one year. If the filter is too much resistance or loss of ability to filter induced normal production, you need to be cleaned or replaced (recommended direct replacement, no cleaning, because after cleaning operations can not reliably guarantee performance of the filter).
1.2 cleaning fermentors, scrub with a soft brush, do not scratch hard to avoid any surface damage fermenter.
1.3 supporting instruments should be checked every year to ensure proper use.
1.4 electrical, instrumentation, sensors and other electrical equipment is prohibited directly with water vapor contact, to prevent moisture.
1.5 devices to stop using, should be promptly cleaned, drained fermenter various pipeline than water; loosen the fermentation jars and hand hole cover screw seal to prevent permanent deformation.
1.6 operating platform, heated tanks and other steel equipment should be regularly (once a year) paint to prevent corrosion.
1.7 If the fermenter temporarily do, you need to eliminate the fermenter were empty, and all side by side to make more than the water tank inside the tubes.
2. Considerations
2.1 single piece of equipment must ensure that all use of the system can run normally.
2.2 sterilization filter, air filter flows through the steam pressure should not exceed 0.17MPa, otherwise the filter cartridge will be damaged, lost filtering ability.
2.3 During the fermentation process, make sure the tank pressure does not exceed 0.17MPa.
2.4 In the real process of elimination, when the jacket through the steam preheat inlet pressure must be controlled within the operating pressure range of the device (no more than 0.2MPa), otherwise it will cause damage to the fermenter.
2.5 Cancellation and empty real consumer, must be drained fermenter jacket remainder water. Doing so may lead to the fermentation tank flattened cylinder, resulting in equipment damage; when the real consumer, but also cause excessive condensate leads broth diluted, making it impossible to process requirements.
2.6 After the air in the cooling process elimination, elimination of solid, non-fermentation tank to produce a negative pressure to prevent pollution, and even damage the equipment.
2.7 in the fermentation process, the pressure tank should be maintained between 0.03 ~ 0.05MPa, in order to avoid contamination.
2.8 In each procedure, you must keep the air pressure in the pipeline pressure is greater than the fermenter tank, otherwise it will cause fermentation liquid back into the filter, clogged filter cartridge or the filter failure.
2.9 If you encounter a problem they can not solve, please contact the company service department. Do not force the demolition or repair.
Various parts of the structure:
Three-part structure: tank and control box, air compressor (Air Station), steam generator (boiler room), mainly on the tank and the control box.
To a stainless steel tank, volume of 30 L,
Tank Structure: The main material is stainless steel 316L tank, the tank no dead ends; seed fermenter with a dedicated sampling pipes, discharge valve, shift. Using a large tank level perspective endoscopic observation, 12V safety depends on light, with temperature, PH, DO, tank top with an exhaust port, a pressure gauge, a safety valve, a foam sensor interface, bacteria inoculated mouth 1, an anti-foaming liquid interface, acid (base) port 1, port 2 feeding, depending on the cap 1
Tank-side interface: air intake, a sampling valve interface, temperature, PH, DO interface for each one.
Tank bottom interface: a discharge port.
Sampling valve, no dead discharge valve design, can be sterilized separately
Tank: Design pressure 0.3Mpa jacket: design pressure 0.35Mpa, optimized diversion design
All metal parts in contact with the material, pipe materials are stainless steel, non-metallic parts require high temperature, corrosion, non-toxic, environmentally friendly, needle valve, ball valve and diaphragm (standard diaphragm, high temperature, acid) can tank Different configurations of the controller performance. The controller can complete the most basic functions, which consists of the following parts:
(1) Parameter Input and display means for control of the fermentation conditions of the various input parameters and display the culture medium during the fermentation tank temperature, pH, DO (dissolved oxygen) measured value.
(2) the electrode correction means for correcting the pH electrode and DO electrodes.
(3) an acid, a base is added to the fermentor pump for acid or alkali to adjust the culture medium of pH.
(4) a defoaming agent was added to the pump defoamer was added to the fermentor in order to eliminate the excessive foam during fermentation.
(5) warning light and buzzer button when the fermentation process, the failure on the circuit, such as the display shows the temperature or DO is flashing, that exceed the measured value of the unit, the alarm red light and issued a "tick, ...... tick "sound.
(6) Automatic or manual control button is in the controller for determining the present automatic control or manual control.
(7) electrically connected to the wire: connection wires are connected with pH, and temperature probe.
Fermenter using mechanical operation
Installed electrodes. Check before sterilization each interface, each line appearance is normal, the steam pipes are secure. They should check whether each valve is in a fully closed state.
Sterilization
Requires at least two people present during sterilization, a person responsible for the operation of various valves, one to observe the parameters of the report.
After confirming that each valve is closed, open the steam generator inlet valve, start the steam generator, steam generator automatically starts the water, you should open the lower part of the steam generator drain valve to the water as possible after release rust close the drain valve . Observe the water level inside the steam generator, to prevent accidents. At this point the valve is fully open valve jacket, slowly open the steam generator of the total valve partially open jacket inlet valve slowly, began to pass into the steam jacket. Vapor pressure at the start may instability, violent swing gauge pointer can be appropriately small jacket off the intake valve, then open the steam valve sampling valve, sampling valve into the steam valve, foot valve out of the steam valve, foot valve into the steam valve for sampling valve and bottom valve sterilization, while water vapor pressure instability drained. No dramatic changes in water vapor pressure when in turn closes the valve bottom valve. Tank temperature gradually rises to 90 ° when the set temperature, stirring motor is stopped, the tank total intake valve opens, open the tank outlet pipe valve, open the following valves: open outlet valve, slowly open the tank into the steam valve to the tank through the steam, air filter gauge begins to rise, the valve opens hydrophobic condensate exceeds 0.1MP, continue to open a large tank into the steam valve, after divisible air tank, the tank pressure to rise slowly, adjusting outlet valve, so as to achieve the desired sterilization temperature and pressure, the start time is reached sterilization temperature. Adjust the jacket inlet valve, the tank into the steam valve and outlet valve, the temperature, pressure tank to maintain the set temperature. Sterilization time to post, and then click Close has opened each inlet valve, close the main steam valve fully open tank exhaust vent let go of the pressure tank, turn off the steam generator power. Open the air compressor, etc. can pressure when dropped 0.05MP, slowly open the intake valve, maintaining a positive pressure inside the tank.
Sterilization process, the operator should wear long trousers with protective gloves to prevent burns. Sterilization process if automatic water steam generator, steam total pressure will drop quickly, if necessary, appropriate to open a large main steam valve, keep the pressure, but be careful observation of the pressure change. When the tank through steam sterilized jar pressure decreased rapidly, attention through sterile air time. Try not to make air compressors and steam generators simultaneously. Air filter pressure sterilization process should not exceed 0.2MP. Will affect the size of the condensate tank after sterilization liquid concentration, to consider increasing the amount of steam condensate.
Ferment
Control of the intake valve exhaust valve can be resized and tank pressure ventilation, control agitation revolutions fermentation dissolved oxygen to meet process requirements. Open fermentation tank inlet valve, open the valve and jacket jacket inlet valve, then the system automatically by controlling the jacket temperature control of fermentation temperature. Due to the positive pressure tank, when inoculated off a small inlet valve, due to lower tank pressure to 0.01-0.02MP, flame biosphere inoculated mouth quickly open (to prevent air flow to the flame goes out), after inoculation inoculation mouth shut quickly. Will be linked into the bottle after each feeding systems and ancillary hose sterilization host (note the direction), pierce silicone mat under fire ring protection installed feeding system. You should have a system to display real-time monitoring of various parameters on the workstation. Note that parameter records do work.
Fermentation late, you may have a bacterial blocked out the trachea, the phenomenon of decreased ventilation, tank pressure slightly increased. At this point man rotation around the valve, you can make jam eased.
FAQs and Troubleshooting
Temperature control failure
Possible Cause: The total tank valve is not open Solution: open a large tank ventilation valve total decline
Possible causes: bacterial clogging the trachea resolved: man rotation vent abnormally high pressure air filter
Possible causes: Air Filters Memory Water Solution: Open the filter traps
Emergency treatment
Power failure
First, turn off the air compressor to prevent sudden power transmission. Turn off the host control unit and the host. Try to maintain the tank pressure and temperature. After power is restored, turn on the host, the control unit and workstations, to be reduced to zero open air compressor pressure air compressor
Sterilization process tailpipe thrown or broken line interface
Trying to close the steam valve, turn off the steam generator, turn off the power. Security personnel evacuated zone, re-cooled exhaust pipe is fixed or replaced accordingly pipelines.
| Company Profile | Technical Support | Technical Support | Links | ||
 | | | | ||
Shanghai Bailun Biological Engineering Co., Ltd. (your side of the fermenter expert) is specialized in the biopharmaceutical industry, R & D and engineering design consulting as one integrated company ... | Culture is the soul of business. Heritage and promotion is the norm hundred gallons corporate culture construction, we use cultural influence thinking, behavior and cultural norms to use, use of cultural cohesion. | Our products mainly for high-end customers, products, materials and devices are high-quality as the premise. From the tank side, I selected stainless steel equipment are .. | Lianyungang Bailun Biochemical Technology Co. |