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  1. Care and Maintenance

1.1 precision filter is generally used for a period of one year. If the filter is too much resistance or loss of ability to filter  induced normal production, you need to be cleaned or replaced  (recommended direct replacement, no cleaning, because after cleaning  operations can not reliably guarantee performance of the filter).

1.2 cleaning fermentors, scrub with a soft brush, do not scratch hard to avoid any surface damage fermenter.

1.3 supporting instruments should be checked every year to ensure proper use.

1.4 electrical, instrumentation, sensors and other electrical  equipment is prohibited directly with water vapor contact, to prevent  moisture.

1.5 devices to stop using, should be promptly cleaned, drained  fermenter various pipeline than water; loosen the fermentation jars and  hand hole cover screw seal to prevent permanent deformation.

1.6 operating platform, heated tanks and other steel equipment should be regularly (once a year) paint to prevent corrosion.

1.7 Check the gearbox oil level frequently, such as lubricating oil is not enough, the need for timely increase.

1.8 reducer regular replacement of oil, in order to prolong its life.

1.9 If the fermenter temporarily do, you need to eliminate the  fermenter were empty, and all side by side to make more than the water  tank inside the tubes.


2. Considerations

2.1 single piece of equipment must ensure that all use of the system can run normally.

2.2 sterilization filter, air filter flows through the steam pressure  should not exceed 0.17MPa, otherwise the filter cartridge will be  damaged, lost filtering ability.

2.3 During the fermentation process, make sure the tank pressure does not exceed 0.17MPa.

2.4 In the real process of elimination, when the jacket through the  steam preheat inlet pressure must be controlled within the operating  pressure range of the device (no more than 0.2MPa), otherwise it will  cause damage to the fermenter.

2.5 Cancellation and empty real consumer, must be drained fermenter jacket remainder water. Doing so may lead to the fermentation tank flattened cylinder,  resulting in equipment damage; when the real consumer, but also cause  excessive condensate leads broth diluted, making it impossible to  process requirements.

2.6 After the air in the cooling process elimination, elimination of  solid, non-fermentation tank to produce a negative pressure to prevent  pollution, and even damage the equipment.

2.7 in the fermentation process, the pressure tank should be maintained between 0.03 ~ 0.05MPa, in order to avoid contamination.

2.8 In each procedure, you must keep the air pressure in the pipeline  pressure is greater than the fermenter tank, otherwise it will cause  fermentation liquid back into the filter, clogged filter cartridge or  the filter failure.

2.9 If you encounter a problem they can not solve, please contact the company service department. Do not force the demolition or repair

 

Stirring mechanism Maintenance:

1.1 the stirring mechanism by a motor, a stirring shaft, stirring blade, the coupling mechanism together constitute. At the upper end of the normal operation of the agitator shaft  couplings using a specially designed, played both the agitator shaft  hang up, but also to ensure the shaft

1.2 stirrer maintenance:

1) Every time after opening the lid should be checked and tightened  installed in various parts of the agitator shaft stopper screw.

2)  bearing the exchange: After two years of use, significantly increased  bearing noise or agitator shaft bearings obvious swing to be replaced. Bearing  cap lifted, lifted head screw shaft and the bearing cap, bearing shed  next door, and remove the bearing retainer at the lower end of the  bearing, taken down by the stirring shaft, remove and replace the  bearing, then turn installation.

Filtered filter changer:

1.1 filter replacement cartridge

Since air contains a lot of dust, after a certain time, pores on the filter will gradually clog. Thus on the one hand caused a serious shortage of air flow and air pressure drop increases, and may cause contamination. At this point it needs to be replaced. Due to large differences over the air quality, so there is no one  exact life, it is recommended sterilization times for 20 batches.

Solenoid valve maintenance:

In ensuring the operation of the circulating pump and circulation line  patency, the temperature did not rise only to drop the biggest reason  is the solenoid valve is leaking or stuck valve

1.1 solenoid valve is used in the fermentation tank cooling, solenoid  valve stuck, damaged valve seals fail and can cause failure of the  solenoid valve solenoid.

1.2 Failure to determine:

Cooling valve failure:

1) Press the water switch, water switch and indicator lights on the  end face of the solenoid valve is non-magnetic, non-cooling water  discharge, bad coil; water switch light is not on, the control box F1  (Realy) fuse burned.

2) off solenoid valve, drain cooling water leakage, seal failure or debris stuck valve poppet;

Other maintenance:

Ball  Maintenance: Because the seal is another ball inside two hemispherical  made into tetrafluoroethylene, after prolonged use, may leak between the  seal and the valve. Maintenance:  fittings at both ends of the valve nut loosen, then tighten the joints  at both ends of the cylindrical valve, you can. (Under normal circumstances: Turn the valve handle should be some resistance)

 

Ferrule fittings leak: to tighten the pressure cap on the connector.

Air hose: To prevent air flow meter is connected to both ends of the  hose burst accident, it is recommended every two years to replace all  the hoses.

Steam generator maintenance: reference steam generator manual.

Air Compressor Maintenance: Manual reference compressor

 

DO use electrodes, care and maintenance

1. Preparation before use

1.2 Unpacking Check the packaging for damage. If the packaging is damaged, please do not continue to open the  packaging, and transportation sectors and immediately Mettler - Contact  TOLEDO, jointly open the package inspection probe for damage after  transport representatives to the scene, the proposed photo evidence.

1.3 However, if the packaging intact probe damage immediately and Mettler - Toledo company. The probe along with the warranty card, manual and original packaging Mettler - Toledo company.

Please read the instructions carefully before using the probe using 1.4. Because dissolved oxygen probe may have stored some time, it is  recommended before first use electrolyte replacement (replacement  method, see 4.2.2).

1.5 DO probe before use electricity more than six hours of polarization (polarization method, see 2.1).

1.6  Safety Warning: DO probe electrolyte is a strong alkaline solution  (pH13), if there is a small amount of electrolyte is spilled on the  skin, wash with water. Electrolyte can not contact with eyes, if the electrolyte into the eyes, immediately rinse the eye with plenty of water. If eye abnormalities (such as redness, pain, blurred vision, etc.), immediately to the hospital.

2. polarization probe

2.1 Polarization Methods: cable and connect the transmitter and the  probe, the probe began after the transmitter is powered polarization.

2.2 The following conditions need to be polarized probe:

◇ probe for the first time, more than six hours polarization;

◇ replace the membrane or electrolyte polarization over six hours;

◇ transmitter power, or probe with cable disconnected polarization time in Table 1;

2.3 polarization time (Table 1)

Sensor / transmitter off time t (min)

Minimum polarization time t (min)

t> 30

360

30≥t> 15

6 × t

15≥t> 5

4 × t

t≤5

2 × t

2.4 polarization voltage

When  measuring high concentrations of the medium (biological fermentation,  waste water treatment), the polarization voltage of -675mV. Measuring low concentrations medium (<500ppb), the polarization voltage is -500mV.

Note 3. When the probe calibration

3.1 Calibration medium can be air or saturated media.

◇ If the air as calibration medium, the probe on the air, water stains  dry film, you can start the calibration When a stable reading.

◇ biochemical fermentation process, and is usually saturated medium for calibration media. After  the real consumer, prior to inoculation, stirring, until the maximum  calibrated when the maximum amount of saturated air pass. Recommended after reunification ventilation calibrated to unify different batches and different fermenter tank saturation.

Must be fully polarized probe calibration before 3.2.

4. Replace the membrane and electrolyte

4.1 In general, we recommend that customers every three months electrolyte replacement operation. But also can decide depending on the circumstances.

4.2 If the electrode signal is not normal (long response time,  mechanical damage, the current increases in oxygen-free medium, etc.),  you need to replace the film.

4.2.1 Replacing steps film (this operation is limited to InPro6000 series, not for Φ12mm dissolved oxygen probe):

1) Disconnect the probe and cable;

2) Unscrew the protective cover film tip of the probe (in this case the dissolved oxygen in the protective film kit);

3) The electrolyte membrane oxygen cylinder down in the waste;

4) The oxygen membrane protective cover (along with oxygen membrane) down on the bench;

5) Take a small flathead screwdriver, with the tip against the Ministry of the protective ring oxygen film.

   Gently force the membrane pressure from the protective sleeve, the  force is perpendicular to the direction slightly tilted outward.

★ Note: The knife is not against the diaphragm, otherwise it will damage the oxygen film.

6) Take a change of dissolved oxygen in the film to be added to the electrolyte. The following operation is the same as 4.2.2 electrolyte replacement


4.2.2 electrolyte replacement operation

1) The residual electrolyte membrane drained, rinsed with deionized water film inside, dry with absorbent paper;

2) the film tilt, the electrolyte vertically downward, as shown in Figure 3a;

3) Gently squeeze bottle of electrolyte, the electrolyte membrane in vivo slow inflow;

4) The amount of electrolyte in Figure 3b;

5) Make sure no air bubbles inside the film, if the bubble can flick membrane, remove air bubbles;

6) The film is set on a rotating body slowly into the electrode, and then carefully tighten the stainless steel casing.


5. Probe current anomalies in the air

5.1  Probe Current in air value refers to a current value of the probe  exposed to the air, generally indicated by the absolute value. Different types of dissolved oxygen probe in air different range of current values, see the probe instruction manual.

Current low 5.2 in the air, the usual causes and treatment methods are as follows:

5.2.1  platinum cathode surface covered with an oxide material, at this time  the head can be observed against the light inside the cathode electrode,  the cathode surface can be seen as black. Available  in more than 1,000 head grade sandpaper gently polished platinum wire  head back and forth several times, as long as the shiny surface of the  platinum wire can be. Must not be over-polished, otherwise inner electrode head will be damaged.

5.2.2 platinum cathode film failed to come into contact with oxygen.

1) Check the oxygen membranes are secured in place, if this is the  case, you should tighten the black protective cover film shown in Figure  1 until the seals do not see so far.

2) Check the oxygen film sheet, if there is excessive protrusion, the  cathode can not come into contact with the membrane, the membrane must  replace the dissolved oxygen.

The current value of 5.3 in the air is high, common causes and treatment methods are as follows:

The reason

Processing method

Probe polarization is not sufficient

Polarization time to confirm compliance with Table 1

Probe deface

Cleaning the probe (deionized water should be used, can not be used cleaning solution containing ethanol)

Electrolyte aging

Electrolyte replacement

Membrane aging or damaged

Replace the membrane

Cable damage

Replace the cable (transmitter should show a low and stable current value when the probe is not connected)

Transmitter damage

Try replacing the transmitter


Save 6. DO probe

6.1 Probe long time without the protective caps should be well placed in the air to save.

 

Use PH electrodes, care and maintenance

1. Preparation before use

1.1 description applies to Mettler - Toledo Instruments (Shanghai) Co., Ltd. Online pH electrode process detection unit sales.

1.2 Unpacking Check the packaging for damage. If the packaging is damaged, please do not continue to open the  packaging, and transportation sectors and immediately Mettler - Contact  TOLEDO, jointly opened the package test electrode is damaged transport  representatives attended, the proposed photo evidence.

1.3 as the packaging intact but electrode damage immediately and Mettler - Toledo company. And electrodes together with the warranty card, manual and original packaging Mettler - Toledo company.

Please read the instructions carefully before using the electrode 1.4 use. If  you have a silicone membrane electrode covered in knife before use  electrodes that comes Carefully scrape silicone, should be directed from  front to back, so as not to scratch the sensitive film. Silicone diaphragm should all pay attention to scrape (until tip hit on a porous ceramic membrane).

1.5 observe whether there is a pH-sensitive membrane electrode bulb  liquid, if not filled with liquid or air bubbles should gently shake the  electrode so that fluid-filled inner bulb, no air bubbles.

1.6  electrodes before first use in acidic buffer (pH4.00) soak for several  minutes, and then in a neutral buffer (pH6.86 or 7.00, etc.) to soak for  several minutes. Then start the calibration.


Note 2. When the electrode calibration

Please note that the use of fresh buffer 2.1 calibration. Such as the use by Mettler - Toledo production buffer when the buffer fade after failure, can not be used. Such as the use NIST series (with user-GB series) buffer, the proposed use within one week after preparation. Refer to the remaining buffer specific instructions for use.

2.2 electrode placed one minute in a buffer before subsequent operations.

2.3 Note to select the correct buffer series, see the meter in the transmitter manual.

2.4 Rinse the electrode with a soft paper towel only water, do not rub the pH-sensitive film.

2.5 electrode calibration cycle, depending on the use of the  environment and precision requirements may be, to determine the  appropriate calibration cycle in ensuring the accuracy of the premise.


Routine maintenance 3. pH Electrode

3.1 General pollution: water, 0.1mol / lNaOH or 0.1mol / lHCl clean the electrode for several minutes.

3.2 fats or organic pollution: clean the electrode for a few seconds with acetone or ethanol.

3.3 sulfide contamination (diaphragm black): The 9892 deal with the cleaning solution (see accompanying instructions 5).

3.4 protein contamination (diaphragm yellow): The 9891 deal with the cleaning solution (see accompanying instructions 5).

3.5 electrode regeneration: regeneration was treated with 9895 (see accompanying instructions 5). Reproducing only a limited extended life of the electrode.

3.6 add electrolyte: For when the electrodes 465 and InPro2000 series Caution time to add electrolyte.

3.7  sheath dwell: for when 465 and InPro2000 series electrodes require the  use of pressurized jacket attention frequently observed on the pressure  gauge readings. In principle, the pressure within the sheath should be higher than the pressure in the reactor 1.5Bar above.

Note: When the measured medium on the electrode sulfide contamination or protein contamination should be promptly treated.

Save 4. pH electrode

4.1 electrode for a long time when not in use should be stored in a suitable specification requirements liquid.

4.2 electrode can not long dry place, can not be stored with the electrode surface during the drying medium. Dry place electrodes should be placed in order to use the appropriate preservation solution after activation.

4.3 InPro3030 / 3100 series pH4 buffer activation, remaining activatable 3MHCl (or saturated KCl) in.

4.4 electrode can not be placed in distilled water saved.


5. electrode processing solution for use

5.1 electrode cleaning solution (9891)

Gastrin / HCl is used to remove protein contamination (diaphragm yellow). Usage: the head of the electrode into the solution, to ensure that the membrane is immersed in a solution (at least 1 hour). Then rinsed with distilled water, re-calibration. Order number: 51340068.

5.2 membrane cleaning fluid (9892)

Thiourea / HCl used to clean black membrane, which is caused by sulfur-containing solution. How  to use: the head electrode immersed in the solution (the membrane into  the solution should not) until the diaphragm colorless (at least 1  hour), then soaked overnight in the 3M KCl, rinse completely,  re-calibration may be used. Order number: 51340070.

 

5.3 electrode regeneration solution (9895)

Sensitive  membrane electrode hand down to the regeneration of droplets in the  sensitive membrane electrode, along the axis of constantly rotating  electrode, it is recommended to keep about 3 minutes, then rinse  thoroughly with deionized water immediately electrode and the electrode  is stored in the specified manual electrode storage solution for 12 hours. Recalibrate after use.